Plant-mediated green synthesis of nanomaterials has been increasingly gaining popularity\ndue to its eco-friendly nature and cost-effectiveness. In the present study, we synthesized silver\nnanoparticles (AgNPs) by using an aqueous solution of Saudi Origanum vulgare L. plant extract as a\nbioreducing agent. The as-synthesized AgNPs were characterized using various microscopic and\nspectroscopic techniques. The results indicated the formation of crystalline face-centered cubic (fcc)\nAgNPs. Additionally, FT-IR study confirmed that the O. vulgare L. extract not only functioned as a\nbioreductant but also stabilized the surface of the AgNPs by acting as a capping agent. Moreover,\nthe effect of the amount of the plant extract on the size and the antimicrobial activity of the NPs was\nalso assessed. It was found that with increasing amounts of plant extract, the size of the NPs was\ndecreased. Moreover, as-synthesized AgNPs as well as O. vulgare L. plant extract were separately\ntested to examine their antimicrobial activities. The activities were tested against various bacterial and\nfungal microorganisms including Shigella sonnei, Micrococcus luteus, Escherichia coli, Aspergillus flavus,\nAlternaria alternate, Paecilomyces variotii, Phialophora alba, and so on. These results evidently show that\nthe inclusion of O. vulgare L. extracts improves the solubility of AgNPs, which led to a significant\nenhancement in the toxicity of the NPs against the assessed microorganisms.
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